JB-VOLUME 02 ISSUE 02 SEPTEMBER 2014


Home JB  Abs.& Indexing  Scope  Archives  Editorial Board  Call for Papers  Submit Papers



Volume 2 Issue 2, September 2014

Journal of Biology

ISSN

2052-0751

Publication Frequency

6 Issues per year

Pages 25-46 Publication History

Original Articles

In vitro Conservation of Sweet Potato under Slow-Growth Conditions with Abscisic Acid

Betty Bazán-Zafra, Consuelo Rojas-Idrogo and Guillermo E. Delgado-Paredes

Pages 25-31


Contamination and Persistence of Salmonella Enteritidis in Stressed and Unstressed Common Carp (Cyprinus carpio L.)

Aleksandra Daskalova, Aleksandar Pavlov and Hristo Daskalov

Pages 32-38


Cytogenetic Assays of Genotoxic Effects and Cancer Risk of Chromium on Tannery Workers in Bangladesh

M. A.Al-Shoeb, M.H. Rahman, S. Chakraborty, M. Faisal, T.M.Z. Waise, F. Hassan, M.F. Kabir and M.A. Hossain

Pages 39-46



Title

In vitro Conservation of Sweet Potato under Slow-Growth Conditi-ons with Abscisic Acid

Abstract

In vitro conservation is especially important for vegetatively propagated and for recalcitrant seed plant species; however, this conventional technology for sweet potato [Ipomoea batatas (L.) Lam.] germplasm collections are laborious due to the need for two and three subculturing procedures per year, which implies high costs and contamination. The aim of this study was to develop a protocol for the in vitro conservation of sweet potato genotypes using the slow growth technique. Apical shoots and nodal segments were stored at 26±2 oC, abscisic acid (ABA) (0.0, 0.1, 0.5, 1.0 and 1.5 mg/L), without 1-Naphthaleneacetic Acid-6-Benzylamino Purine (NAA-BAP) or with 0.18 mg/L NAA-BAP (0.22 and 0.67 mg/L). In additional experi-ment, explants were stored with ABA (0.0 and 0.5 mg/L) and sucrose (2.0, 4.0 and 6.0%). After six months of storage, plantlets were evaluated for shoot elongation, number of nodes, leaves, buds and roots formation, survival and viability (regro-wth). In vitro cultures maintained on MS medium supplemented with ABA (0.5 and 1.0 mg/L) and 0.18 mg/L NAA-0.22 mg/L BAP combination showed 50 to 70% survi-val with slow-growth shoots.  

Keywords

Germplasm Bank, Growth Inhibition, Micropropagation, Sucrose Concentration, Sweet Potato


Title

Contamination and Persistence of Salmonella Enteritidis in Stressed and Unstressed Common Carp (Cyprinus carpio L.)

Abstract

Salmonella is an important foodborne pathogen, causing a lot of different clinical syndromes and contamination of the human food can be a significant public health concern. The aim of this study was to assess the ability of Salmonella Enteritidis to penetrate and persist into the tissues of live common carps and the influence of stress on this ability. Four groups of fish were tested: control group, group of fish injected intraperitoneally with S. Enteritidis, group of unstressed carps and group of oxygen-deprived carps both immersed in water containing S. Enteritidis. For assessment of the stress level blood cortisol and glucose concentrations were meas-ured. Carps, subjected to reduced dissolved oxygen concentration for four days, showed higher cortisol (P ≤ 0.05) and glucose (P ≤ 0.001) levels (283.46173.49 ng mL-1 and 6.74±0.93 mmol L-1, respectively) compared to the unstressed fish (74.81 ±46.51 ng mL-1 and 3.61±0.90 mmol L-1, respectively). Intraperitoneally injected carps carried Salmonella up to 16 days after the inoculation. Stressed and unstress-ed fish contaminated with S. Enteritidis by immersion showed presence of the path-ogen on the skin and gills 24 hours after the infection, but was not isolated at 4, 8 and 11 days. No difference was found in Salmonella persistence between these two groups.

Keywords

Stress, Common Carp, Salmonella Enteritidis, Contamination, Food Safety


Title

Cytogenetic Assays of Genotoxic Effects and Cancer Risk of Chromium on Tannery Workers in Bangladesh

Abstract

Chromium is a trace element for human body, and it’s role as a potent cytogenoto-xic agent is well documented. Cr (VI) is considered as a human lung carcinogen wi-th potentially widespread exposure. As different Chromium compounds are used in tanneries, the workers are at a risk of potential occupational exposure to chrom-ium. This study was designed to evaluate cytogenotoxicity of chromium compounds used in leather industries on human peripheral blood lymphocytes by using a series of cytogenotoxic assays such as cytokinesis-block micronucleus (MN) test, chromoso-me aberration (CA) test and alkaline Comet assay. The MN assay revealed a statisti-cally significant (p<0.001) increase in the frequency of micronucleus in peripheral blood lymphocytes of exposed groups (A, B & C) and CA assay also shows the signifi-cant increased value of chromosome aberration 10.27±0.4933, 16.70±0.9849 and 28.80±1.114 of exposed groups. The Comet assay also indicated the significant inc-rease in Comet tail DNA (%), tail moment and Olive tail moment. These results provide evidence that the duration of exposure to chromium compounds in human peripheral lymphocytes results in induction of DNA damage. Therefore this study suggested that chronic occupational exposure to Cr could lead to increased levels of DNA damage and chromosomal instability.

Keywords

Chromium, Cytogenotoxic, Lymphocyte, Micronucleus Test, Chromosome Aberration, Comet Assay