RJB-VOLUME 02 ISSUE 05 SEPTEMBER 2012


Home JB  Abs.& Indexing  Scope  Archives  Editorial Board  Call for Papers  Submit Papers

Volume 2 Issue 5, September 2012

Research Journal of Biology

ISSN

2049-1727

Publication Frequency

6 Issues per year

Pages

138-167

Publication History



Original Articles

Antimicrobial Activity of Ocimum basilicum and its Inhibition on the Characterized and Partially Purified Extracellular Protease of Salmonella typhimurium

S.A. Adeola, O.S. Folorunso and K.O. Amisu

Pages 138-144


Study of Reproductive Traits of Spiny Eel, Mastacembelus armatus (Mastacembeliforms) from Kalinadi-A Tributary of the Ganges River Basin, India

M. Serajuddin and B.C. Pathak

Pages 145-150

 

Malaria and Working Performance of Academic Staff in a Nigerian University

O.O. Okwa, L.O. Sanyaolu and A.F. Olatokunbo

Pages 151-156


Fish Diversity in Two Lakes of Kumaon Himalaya Uttarakhand, India

Ram Krishan Negi and Vishal Rajput

Pages 157-161

 

The Seedling Emergence Fitness of Diclafop Methyl Resistant and Susceptible Lolium rigidum as Affected by Temperature and Planting Depth

Mona Dastoori, Hamid R. Mashhadi, Saeed Shahbazi, Malek Yazdani, Hesam E. Imouradabi and Ali Lashkari

Pages 162-167

 



Title

Antimicrobial Activity of Ocimum basilicum and its Inhibition on the Characterized and Partially Purified Extracellular Protease of Salmonella typhimurium

Abstract

The volatile oil of Ocimum basilicum, at the preliminary stage, showed a wide range of antibacterial activity (10.5±0.5 to 19.5±0.5 mm) against thirteen different enteric bacteria with highest sensitivity against Pseudomonas aeruginosa but Providential alcalifaciens and Providential rettgeri were resistant to the oil. The minimum inhibitory/bactericidal concentrations (MIC/MBC) results showed the oil to inhibit Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli at 50%v/v. The MIC for most of these pathogens ranged from 3.12-25%v/v, while Proteus vulgaris and Salmonella typhimurium was 1.56%v/v. Citrobacter fruendii has the lowest value, 0.39%v/v. The MBC of the volatile oil was within one twofold dilution of MIC for each organism. The crude enzyme had optimal activities at pH and temperature of 7.9 and 44o C respectively. The oil showed a noncompetitive inhibition against the activity of the extracellular protease of Salmonella typhimurium and apparently decreased reaction rate from 5.6 x 103 μmol/min (absence of inhibitor) to 3.6 x 103 μmol/min (presence of inhibitor), while the Km remained 2.2 mg/ml. Purification fold of 6.06 and enzyme activity of 382.0 µmol/min as compared to the crude extract were achieved. The volatile oil of Ocimum basilicum, therefore, exhibited a wide range of antibacterial activity and showed a noncompetitive inhibition against the extracellular protease of Salmonella typhimurium.

Keywords

Ocimum basilicum, Volatile Oils, Salmonella typhimurium, Extracellular Protease

Title

Study of Reproductive Traits of Spiny Eel, Mastacembelus armatus (Mastacembeliforms) from Kalinadi-A Tributary of the Ganges River Basin, India

Abstract

The population of Mastacembelus armatus was slightly dominated by males. Sex ratio varied with size and change in season. Monthly fluctuations in gonadosomatic index were recorded and five stages of gonad maturity in both sexes were identified. Ova diameter revealed the release of only one batch of mature ova during the breeding season, which extended from late June to early September. The relative fecundity was 63 eggs cm-1 body length and 29 eggs g-1 body weight. Four predictors of fecundity, body length, body weight, and ovary length and ovary weight were evaluated and regression equations derived. Fish weight proved to be the most precise predictor of fecundity. Absolute fecundity varied between 927-7409 in the specimens of M. armatus ranging between 12.0-48.2 cm in size.

Keywords

Fecundity, Gonadosomatic Index, Sex Ratio

Title

Malaria and Working Performance of Academic Staff in a Nigerian University

Abstract

Malaria remains a social public health problem today. The effect of malaria on the working performances of randomly selected Academic Staff (A.S.) of the Lagos State University (LASU) in South-Western Nigeria was investigated. Structured questionnaires were administered to randomly selected A.S. comprising of 40 Females and 50 Males. Unstructured questionnaires were administered to 10 Staff Doctors (S.D.) as a second exploratory approach. The results indicated that for both gender the history of the frequency of illness, time loss, hospital admission were similar. Similarly, inefficient delivery of lectures was the most reported adverse effect and Insecticide Treated Nets (ITNS) were the best preventive measure.  However, differences existed in methods of seeking treatment, drug use and amount spent on malaria between genders. Staff doctors reported that an average of 21 A.S. visited the health centre daily of which 17 (81%) are often diagnosed for malaria, while 6 (1.1%) may be admitted monthly. They also indicated that more Female staff (76.1%) reported than Male staff. We give the detail of how malaria affects and impacts even on some highly educated people with adequate knowledge of Malaria. The negative effect on working performance is a driving force for prompt and early treatment of malaria. This reiterates the fact that malaria is everybody business, no matter the educational level and status.

Keywords

Academic Staff, Malaria, Working Performance, Lagos State University, Insecticide Treated Nets

Title

The Seedling Emergence Fitness of Diclafop Methyl Resistant and Susceptible Lolium rigidum as Affected by Temperature and Planting Depth

Abstract

Greenhouse and laboratory studies were conducted in 2009 and 2010 to compare the relative fitness of diclafop methyl-resistant Lolium rigidum accessions with their diclafop methyl -susceptible counterparts. Treatments were included seedling emergence of Lolium populations at constant temperatures (5, 10, 15, 20 and 25 ºC) and sowing depth (0, 2, 4, 6 and 8 cm). At 15 ºC with increasing temperature percentage emergence was increased, where the S biotype exhibited a higher percentage emergence compared to two other biotypes. Also, the maximum emergence rate for S biotype occurred at 21 ºC, in contrast, the maximum emergence of the R and semi-R biotypes occurred when their seeds were placed at 18 ºC. The maximum emergence percentage of the R and S biotypes occurred when their seeds were placed at a 6.28 cm depth, while maximum emergence of the semi-R biotype occurred when its seeds were placed at a 5.46 cm depth. The R and S biotypes showed 70% emergence while semi-R biotype showed 60% emergence when seeds were sown at a depth of 8 cm. Rate of emergence in three populations (R, semi-R and S) increased as depth increased. Maximum and minimum rate of emergence for the S biotype was greater compared to R and semi-R biotypes.

Keywords

Biotype, Temperature, Fitness, Sowing Depth, Lolium rigidum