IRJP-VOLUME 02 ISSUE 06 NOVEMBER 2012


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Volume 2 Issue 6, November 2012

International Research Journal of Pharmaceuticals

ISSN

2048-4143

Publication Frequency

6 Issues per year

Pages

148-169

Publication History


Original Articles

Gentamicin, Salicylate and Their Combinations as Anti-Infective Coating of Orthopedic Implants

Gamal Fadl M. Gad, Adel A. Abdel Aziz and Reham A. Ibrahem Aly

Pages 148-154


Controlled Solidification of Gellan Gum Using Macro Nutrient Solution as a Trigger

Anuja Bapat, Dhanashri Mungekar, Vijay Thorve, Shubhangi Jhadav and Pratap N. Mukhopadhyaya

Pages 155-160


Prophylactic Efficacy of a Combination of Proanthocyanidin and Vitamin E on Hepatotoxicity Induced by Doxorubicin in Rats

Noorah Saleh Al-Sowayan and Uday Kishore

Pages 161-169

  


Title

Gentamicin, Salicylate and Their Combinations as Anti-Infective Coating of Orthopedic Implants

Abstract

Our objective was to determine the efficacy of gentamicin, salicylate and their combinations- PDLLA-coated orthopedic stainless steel wires in preventing gram positive and gram negative bacterial colonization. Stainless steel wires were coated with gentamicin/salicylate combinations. The zone of inhibition (ZoI) around the wires with and without drugs was determined by a modified Kirby–Bauer method. The adherence of bacteria to coated and uncoated wires and after 3 months shelf life followed by 4 weeks of immersion in serum were determined. Gentamicin/salicylate coated metal segments showed the highest inhibitory effect on bacterial adherence when compared with controls (93.1%–100%). These segments showed zones of inhibition ranged from (20-45 mm) diameter. After 4 weeks immersion in serum, the coated wire segments maintained significant durability and inhibited bacterial adherence when compared with controls by 92.8%-93.7%. Salicylate increases the coating efficacy of gentamicin when used in combination with them. These combinations can be used as coating for the orthopedic implants due to their effect in preventing biofilm formation.

Keywords

Gentamicin, Orthopedic, Salicylate, Biofilm, Implants

Title

Controlled Solidification of Gellan Gum Using Macro Nutrient Solution as a Trigger

Abstract

The effect of different components of plant tissue culture media in assisting solidification by Gellan Gum was explored. Macro nutrients comprising of ammonium nitrate, calcium chloride, magnesium sulphate, potassium phosphate and potassium nitrate were found to be collectively important in the gelling process. Mean gelling time was 10 (±0.7) minutes after MS-macro was brought in contact with the antibiotic mixed macro nutrient solution. This rose to 15 (±0.5), 12 (±0.8), 13 (±0.7), 20 (±0.8) and 21 (±0.9) minutes when calcium chloride, potassium nitrate, magnesium sulphate + calcium chloride, magnesium sulphate + calcium chloride + ammonium nitrate and potassium phosphate + magnesium sulphate + calcium chloride + ammonium nitrate were omitted from the media respectively. A modified operating procedure for triggering gel formation was demonstrated by adding pre-sterilized MS-macro media with pre sterilized macro nutrient solution present inside tissue culture bottle and freshly amended with antibiotic at room temperature. The effectiveness of the method in controlling tissue culture contamination is demonstrated. The advantage of Gellan Gum as a cation-sensitive gelling agent capable of generating a transparent tissue culture medium at room temperature is discussed.

Keywords

Controlled Solidification, Gellan Gum, Macro Nutrient Solution

Title

Prophylactic Efficacy of a Combination of Proanthocyanidin and Vitamin E on Hepatotoxicity Induced by Doxorubicin in Rats

Abstract

Doxorubicin (Dox) is a common chemotherapeutic anticancer drug. One of its side effect, according to some studies is oxidative stress-induced toxic changes on the liver. In this study protective efficacy of Proanthocyanidin (GSPE) along with vitamin E on Dox induced hepatotoxicity was evaluated in 50 male (divided in to five groups) Wister rats. After 2 weeks of the experiment and drug administration, blood samples and liver tissues were taken from all groups. Lipid profiles, liver function test in blood serum and Superoxide Dismutase (SOD), Catalase (CAT) and Malondialdehyde (MDA) levels in liver tissue were measured. We found that intraperitonial administration of Dox had profound effects on the liver as assessed by liver function tests and liver histology. The present results showed that pretreatment with proanthocyanidin extract, along with vitamin E enhances the antioxidant defense against Reactive Oxygen Species and reduced the oxidative stress and change in activity of antioxidant enzymes induced by Dox, thereby demonstrating the antioxidant potential of GSPE along with vitamin E in ameliorating the hepatotoxicity induced by Dox and it shows that it could be one approach to reduce the risk of Dox induced side effects in clinical settings.

Keywords

Dox, Oxidative Stress, Hepatotoxicity, Reactive Oxidation Species, Antioxidant Enzymes, Lipid Peroxidation, Proanthocyanidin, Vitamin E