RJB-VOLUME 02 ISSUE 06 NOVEMBER 2012


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Volume 2 Issue 6, November 2012

Research Journal of Biology

ISSN

2049-1727

Publication Frequency

6 Issues per year

Pages

168-196

Publication History


Original Articles

Inducing HL-60 Cell Differentiation Using Maltese Plant Extracts

Andrei Agius Anastasi, Analisse Cassar and Pierre Schembri-Wismayer

Pages 168-176

A Chromosome Count in Marama Bean (Tylosema esculentum) by Feulgen Staining using Garden Pea (Pisum sativum L.) as a Standard

M. Takundwa, P.M. Chimwamurombe, and C.A. Cullis

Pages 177-181

Study of Effect of Hypothyroidism on Platelet Aggregability

Dr. Anita Chaudhary, Dr. Kamlesh Jha and Dr. T.S. Chaudhary

Pages 182-185

Seasonal Variation in Monogenoid Abundance on Puntius sp.

Ritika Raj Shrivastava, Girdhar G. Agrawal and Nirupama Agrawal

Pages 186-190

Portable Battery-Operated Rapid PCR Amplification of the CAG Repeat Region of the Huntington Disease Locus

Jennifer M. Love, Renate Marquis-Nicholson, Rachel C. Love, and Donald R. Love

Pages 191-196



Title

Inducing HL-60 Cell Differentiation Using Maltese Plant Extracts

Abstract

Despite the extensive research into the pathogenesis and treatment of leukaemias, mortality rates from the more aggressive types have remained distressingly high. The revolutionary introduction of ATRA (All-Trans Retinoic Acid) as the treatment of acute promyelocytic leukaemia, previously known to have an invariably dismal prognosis, has managed to achieve a relapse-free survival of about 90% in these patients, springing a new wave of research in the direction of differentiation of leukaemic cells. With this in mind, the aim of this research is to test and identify different plant extracts that will induce the differentiation of retinoic acid resistant HL-60 cells. Any of these, which are active, can provide sources for further research into possible lead compounds for treating Acute Myeloid Leukaemia, not responsive to ATRA. For this project, plant extracts taken from two indigenous species on the Maltese islands (Darneilla melitensis and Senecio bicolor) have been used. The differentiation of cells incubated with a range of plant extract concentrations (with or without retinoic acid as an adjuvant compound) was measured using spectrophotometry assessing the enzymatic reduction of Nitro-Blue Tetrazolium (NBT) by the mature cells. Promising result of our study encourages further research into these and other plants for possibly new differentiation-inducing agents for leukaemias and even solid tumours such as astrocytomas. 

Keywords

Darniella melitensis, Senecio bicolor, HL-60 Cell Differentiation, ATRA, Acute Promyelocytic Leukaemia

Title

Study of Effect of Hypothyroidism on Platelet Aggregability

Abstract

The chromosome number and the amount of cell space it takes up is one measure of the amount of genetic material in a cell. To determine the chromosome number in marama bean (Tylosema esculentum), the Schiff’s or Feulgen staining technique was used together with oil immersion microscopy under the 100X objective to obtain images from which chromosome counts could be made. The chromosome number and general size of chromosomes in marama bean were determined and compared with those in garden pea. Chromosomes in marama were found to be more numerous than in garden pea though smaller in size. In Tylosema esculentum the chromosome number was found to be n=22 (2n=44) and in Pisum sativum it was the expected n=7 (2n=14). It is postulated that the genome size of marama is smaller than that of common pea since the cell space occupied by the 44 chromosomes is less than the 14 in garden pea. Genome wide next generation sequencing is underway to investigate this suggestion about the genome size of marama bean.

Keywords

Chromosome Count, Marama Bean (Tylosema esculentum), Feulgen Staining, Garden Pea (Pisum sativum L.)

   

Title

Study of Effect of Hypothyroidism on Platelet Aggregability

Abstract

Hypothyroidism is a common condition in which there is multisystem derangement of physiological functions due to lack of thyroid hormone. Various changes in the haemostatic profile have been described in patients with excess and deficiency of thyroid hormone. These changes could be encountered as complicating factors in various systemic disorders like cardiovascular disorders, endocrinal disorders and gynecological disorders like menorrhagia etc. The present study is focused upon one of the haemostatic parameter that is platelet aggregability in clinically confirmed hypothyroid population. As women are affected approximately six times more frequently than men and to avoid any possible gender bias all subjects taken for the study were female hypothyroid patients. Measurement of platelet aggregability was done by using ‘O’ Brien method. Result shows significant increase (P<0.05) in platelet aggregability in the study population. The study provides possible mechanism in thyroid dysfunction which modifies physiological process of primary and secondary haemostasis leading to bleeding or thrombosis as complication.

Keywords

Hypothyroidism, Haemostasis, Platelet Aggregability

Title

Seasonal Variation in Monogenoid Abundance on Puntius sp.

Abstract

Three species of fish Puntius Hamilton, 1822: P. sophore (Hamilton, 1822), P. ticto (Hamilton, 1822) and P. chola (Hamilton, 1822) collected from river Gomti at Lucknow, India, were examined during Jan 2010-Dec 2011. The seasonal changes (Summer, Winter and Monsoon) in abundance of two monogenoidean genera Dactylogyrus Deising, 1850 (13 species; 4 known and 9 unknown) and Dactylogyroides Gusev, 1963 (4 species), highly host specific, were recorded. Some species occur in warmer months while the others in cold (though the survival conditions are worsened), indicating wider range of temperature tolerance. Occurrence of monogenoids show an increasing or decreasing trend with rise and fall in temperature in general, but in the present study, an additional seasonal effect in different calendar periods was observed in parasitic infection on Puntius sp.

Keywords

Seasonal Variation, Monogenoidea, Abundance, Peaks

Title

Portable Battery-Operated Rapid PCR Amplification of the CAG Repeat Region of the Huntington Disease Locus

Abstract

PCR amplification of human DNA is a pivotal technique in clinical molecular diagnostics. Critically, there is increasing interest in developing cheap but robust PCR amplification platforms that allow testing to be done under conditions of unreliable power, or with a need for portability. We have amplified the CAG repeat region of the human Huntington Disease gene in a number of patients referred for diagnostic testing using a commercially available battery operated PCR platform. This work tested the feasibility of amplifying increasing amplicon lengths using a rapid predefined cycling protocol, as well as evaluating the usefulness of this platform for repeat-primed PCR.

Keywords

Convective PCR, Repeat-Primed PCR, Huntington Disease