JB-VOLUME 01 ISSUE 02 MARCH 2013


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Volume 1 Issue 2, March 2013

Journal of Biology

ISSN

2052-0751

Publication Frequency

6 Issues per year

Pages

39-60

Publication History


Original Articles

Gastrointestinal Parasites of the Roucktail Rock Agama, Laudakia stellio from Gaza Strip, Palestine                                                                                

Abdelnasser Rayyan, Basam Al-Zain and Adnan Al-Hindi

Pages 39-41

Gonado-Somatic Index, Sex Ratio and Fecundity of Tilapia zilli in a Tropical Reservoir, South West Nigeria

J.A. Oso, O.A. Ogunleye, E.O. Idowu and F.A. Majolagbe

Pages 42-45

Development of Callus Initiation and Regeneration System of Different Indigenous indica Rice Varieties

Kazi Muhammad Ahasanul Hoque, Z.A. Azdi and Shamsul H. Prodhan

Pages 46-51

Transcriptomic Analysis Reveals Stress Conditions of Human Gastrointestinal Conditions on Saccharomyce cerevisiae

Walid A.M. Omara, Lubomira Stateva and Steve Oliver

Pages 52-60


Title

Gastrointestinal Parasites of the Roucktail Rock Agama, Laudakia stellio from Gaza Strip, Palestine.                                                              

Abstract

Palestine is located to the east of the Mediterranean Sea between 29 and 33 North latitude 35 and 39 longitude, the West Bank and Gaza Strip are two regions in Palestine. Parasitological examination of the gastrointestinal tract of 67 roucktail rock agamas (Laudakia stellio) from the Gaza Strip, Palestine recovered 2 species of nematodes, Parapharyngodon bulbosus and Strongyluris calotis, 1 species of cestode Oochoristica tuberculata and 1 species of ciliated protozoan, Nyctotherus hardwickii. Prevalence of P. bulbosus was (100 %), S. calotis (67 %), O. tuberculata (78 %) and N. hardwickii (90 %). Mean intensity ± SD and ranges were P. bulbosus 40.5 ± 24.2, (1-115 ), S. calotis 34.3 ± 21.6, ( 0-74 ); O. tuberculata 1.5 ± 0.5, ( 0-2 ). Nyctotherus hardwickii and O. tuberculata are reported for the first time from the Gaza Strip, Palestine.

Keywords

Lizards, Laudakia stellio, Agamidae, Nematoda, Cestoda, Protista, Prevalence, Intensity, Gaza Strip, Palestine


Title

Gonado-Somatic Index, Sex Ratio and Fecundity of Tilapia zilli in a Tropical Reservoir, South West Nigeria

Abstract

Some aspects of reproductive biology of Tilapia zilli in Ero Reservoir were studied. Fish samples were collected from the fishermen between April and December, 2009 using gill netting and cast netting. 81 specimens collected were taken to the laboratory for gonado-somatic index, sex identification and fecundity studies. There was no matured ovary found for fecundity determination and the standard length in males ranged from 10.60-20.70 cm and 11.00-20.70 cm in females. The mean gonado-somatic index was 0.6 ± 1.0 and sex ratio was approximately 1:3 (males: female). Spawning was seasonal and more in the wet season through to September and seemed to be triggered off by either rainfall or low temperature. The analysis shows a high, positive and significant relationship between fish weight and standard length, and fish weight and weight of gonad (P< 0.05). On the other hand, there exist a weak and positive relationship between fish weight and GSI, standard length and GSI, although significant at 0.05 levels.

Keywords

Tilapia zilli, Gonado-somatic Index, Fecundity, Sex Ratio, Ero Reservoir


Title

Development of Callus Initiation and Regeneration System of Different Indigenous indica Rice Varieties

Abstract

The aim of the present study was to develop an efficient protocol for best callus induction and complete plant regeneration for varieties of rice (Oryza sativa) i.e. Pakhi Biroin, Hati Baromashi, Kacha Biroin, Badal Boro, Porichok Amon, Khoiya Boro, Joria Aman and BRRI Dhan53. Pakhi Biroin, HatiBaromashi, BadalBoro, Porichok Amon, KhoiyaBoro and Joria Aman cultivate mainly in Sylhet & Sunamgonj district of Bangladesh. For callus induction from mature dehusked rice seeds MS (Murashige & Skoog, 1962) media supplemented with different concentrations i.e. (1.5, 2.0, 2.5, 3.0 mg/L of 2,4-D (2,4 dichlorophenoxyacetic acid)) were used. Khoiya Boro and Joria Aman did not show any efficiency in callus induction. Maximum percentage of callus induction efficiency that is 100% was noted for Pakhi Biroin, Hati Baromashi and BRRI Dhan53, but 75% was the highest percentage of callus for Kacha Biroin. For plantlet regeneration of Pakhi Biroin, Hati Baromashi and Kacha Biroin, MS media with 0.5 mg/L NAA (1-Napthaleneacetic acid), 0.5 mg/L Kinetin and various concentrations of BA (6-benzyladenine) (1.0, 2.0, 3.0, 4.0 mg/L) were employed. On the other hand, MS media with 1.0 mg/L NAA, 1.0 mg/L Kinetin and various concentrations of BA (1.0, 2.0, 3.0, 4.0 mg/L) were used for BRRI Dhan53 regeneration. Different result was recorded for different varieties at various hormone concentrations.

Keywords

Mature Embryos, Genotypic Variability, Embryogenic Callus, Plant Regeneration, Indica Rice


Title

Transcriptomic Analysis Reveals Stress Conditions of Human Gastrointestinal Conditions on Saccharomyce cerevisiae

Abstract

Yeast has been used for the oral application of vaccines and drugs because it is relatively stable, non-pathogenic and non-invasive in the gut compared to other microbial delivery systems. However, been used orally, yeast will face new challenges represented by the Gastrointestinal conditions which include several stress conditions such as heat shock, pH, oxidative and osmotic stress. These conditions will not only affect yeast behaviour but also affect yeast expression and secretory systems as well. Although several previous studies have investigated the effect of different stress conditions on yeast behaviour, most of them considered the effect of single stress factor. None of them investigated the multiple stress conditions found in the human gastrointestinal tract. This study is the first to investigate the effect of such complex conditions on the yeast. Genome-wide transcriptional analysis was performed to show the response of the yeast cells to various stresses in the human gut including heat shock, pH, oxidative, and osmotic stress. Genes showing response to these stress conditions were clearly represented.

Keywords

Yeast, Stress, Gastrointestinal Tract, Saccharomyce cerevisiae