JB-VOLUME 01 ISSUE 04 JULY 2013


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Volume 1 Issue 4, July 2013

Journal of Biology

ISSN

2052-0751

Publication Frequency

6 Issues per year

Pages

86-99

Publication History


Original Articles

Effect of Cage Type and Flooring on Reproductive Performance of Laboratory Rats

K.H.M. Ashoka Deepananda

Pages 86-91


Isolation, Similarities and Subcellular Localisation of Transaldolase from Sugarcane (Saccharum officinarum)

Allegendran Rajeendran, R. Nulit, Nahid Kalhori and Christina Yong Seok Yien

Pages 92-99


Title

Effect of Cage Type and Flooring on Reproductive Performance of Laboratory Rats

Abstract

Present study evaluated the behavioural and physiological measures of welfare on preference of female Wistar rat to litter in two types of solid bottom cages and a grid floor cage. In this regard, reproductive indices and fetal findings were measured, analyzed and compared. Higher reproductive performances were observed in rats tested in solid bottom cages compared to grid floor cages. Pre- and post-implantation losses was significantly (p<0.05) lower in two types of solid bottom cages, while those of in grid floor cages were significantly (p<0.05) higher. Moreover, gestation index, litter index, number of littered pups and mean pups weights were significantly higher (p<0.05) in rats tested in solid bottom cages. Of the two types of solid bottom cages, better reproductive performances were observed from the animals kept in solid round bottom cages through which lowest amount of light could reach animals at daytime. Present findings therefore suggest that more attention should be given to cage type and flooring when maintaining laboratory animals. Also, laboratory animals should be reared in solid bottom cages with sufficient floor area in order to ensure better welfare and grid floor cages should be avoided to achieve the best results from the animal experimentation.

Keywords

Cage Type, Flooring, Laportamy, Reproductive Indices, Welfare, Wistar Rat


Title

Isolation, Similarities and Subcellular Localisation of Transaldolase from Sugarcane (Saccharum officinarum)

Abstract

Transketolase (TK) is one of the key enzymes that involved in the Oxidative Pentose Phosphate Pathway (OPPP), and produce erythrose-4-phosphate which is the precursor for many secondary metabolites such as aromatic amino acids, lignin and flavonoid. The OPPP is composed of two functionally-connected phases, the oxidative and non-oxidative phase. The complete OPPP is localized in cytosol of animal and prokaryotic. However, in plant, the first phase is localised in cytosol but the sub-cellular localization of the second phase of OPPP is still under debate. There is no study available on transketolase in Amaranthus tricolor till to day? Therefore, the objectives of study are to isolate TK gene from Amaranthus tricolor, to compare its identity with other plant species and to determine its sub-cellular localization. The full length of 2021bp nucleotide sequence of TK had been isolated from A. tricolor by RT-PCR. ClustalW revealed that A. tricolor TK sequences showed high similarity (more than 81 %) within plants’ other species. Subcellular localization by using TargetP 1.1 and ChloroP revealed that of A. tricolor TK was located in the plastid. Thus it can be concluded that the OPPP is incomplete in the cytosol.

Keywords A. tricolor, Transketolase, Oxidative Pentose Phosphate Pathway, Subcellular Localization